Multi-residue analysis for the
determination of pesticide residues in fruit and vegetables using liquid
chromatography coupled with mass spectrometry
Corina Mihaela OPRITA1,2,
Nela LUPU2, Marilena CANUTA2,
Natalia ROȘOIU1,3,4
1
Institute of PhD Studies, Doctoral School of Applied
Sciences Ovidius University Constanta, email cioara.corina-ct@ansvsa.ro
2 Specialist Engineer at the Veterinary
Sanitary Laboratory and for Food Safety Constanta;
3
Prof.Univ.Emeritus PhD
Ovidius University, Faculty of Medicine
4Academy
of Romanian Scientists
Abstract. This article summarizes the results of validation studies of a
multi-residue method for the determination of pesticide residues in fruits and
vegetables. The results of some of the monitoring control programs carried out
in Romania are also presented. Liquid chromatography coupled with mass
spectrometry (LC-MS / MS) was used to assess pesticide residue levels. The
QUECHERS extraction method was used to extract the compounds. To validate the
method, the control samples were fortified with a solution of 74 pesticides for
LC-MS / MS analyzes, at 3 levels.The
validation study is based on the regulations in Document DG N SANTE /
12682/2019. The scope of validation included the following performance
requirements: Limit of detection; Quantification limit; Recovery; Fidelity;
Repeatability; Reproducibility; Recovery; Selectivity / Specificity; Stability
/ Robustness; Linearity; Matrix effect; Measuring the margin of error.
Key words: pesticides in fruits and vegetables,
chromatographic liquid analysis, mass spectrometry, food safety
DOI https://doi.org/10.56082/annalsarscibio.2022.1.65
